Measurement

Part:BBa_K549027

Designed by: Franziska Haefele   Group: iGEM11_LMU-Munich   (2011-09-16)


10x His-tag fused to petE2

This BioBrick contains the 10x His-tag and the gene petE2 coding for plastocyanin. The backbone is the expression vector J04500.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal PstI site found at 236
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 236
    Illegal NotI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 434
    Illegal BglII site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal PstI site found at 236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal PstI site found at 236
    Illegal NgoMIV site found at 23
    Illegal AgeI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None